PATHOLOGY

Supervisor Muscle Pathology (Institute of Pathology)
Prof. Dr. Martin Lammers, neuropathologist 
Tel: +31 24 3614314

Technical assistance and secretary:
Henny Kuppen
Lilian Eshuis
Marianne Blokpoel-de Ruyter
Monique van Asseldonk
Tel: +31 24 3615298

General information:
The muscle pathology laboratory is part of the Department of Pathology. It is involved in diagnostics and research concerning neuromuscular disorders.

Laboratory tests:
Routinely enzymehistochemical and morphometric examination is performed on frozen muscle samples. The following stains are routinely performed: HE, Gomori trichrome , Sudan Black, PAS, acid phosphatase, ATPase at pH 4.2, 4.6 and 10.3, NADH, SDH and COX,. Based on the initial findings the examination is completed with immunohistochemistry and electron microscopy.

Shipping and Handling:
The material for enzyme and immunohistochemistry must be snap frozen in isopentane -140 C chilled in liquid nitrogen. When frozen this way it can be sent on dry ice.
For electron microscopy fixation in 2 % buffered glutaraldehyde is necessary.

Reporting of results:
A preliminary report is available at the day following the biopsy. A final report is sent out as soon as all the test results have been obtained. This is routinely within 3 weeks from the date of receipt of the request form with material.

Billing:
In accordance with Dutch regulations the costs are 50 Euro (110 Dfl), 180 Euro (400 Dfl) for enzymehistochemical examination, 116 Euro (255 Dfl) for morphometric examination, 180 Euro (400 Dfl) for immunohistochemical examination and 160 Euro (290 Dfl) for electron microscopy.